Difference Wiki

Kinase vs. Phosphorylase

The main difference between the Kinase and Phosphorylase is that Kinase catalyzes the addition of phosphate from the ATP to acceptor, whereas Phosphorylase from inorganic phosphate (Pi).

Key Differences

Kinases are only regulated by phosphorylation; on the other hand, phosphorylases are regulated by both allosteric as well as phosphorylation.
Samantha Walker
Apr 28, 2020
Kinases add the phosphate from ATP to the substrate, while phosphorylase adds phosphate from the inorganic phosphate to the substrate.
Kinases do not involve the bond-breaking, whereas phosphorylase involved the bond breaking in the substrate molecules.
Kinase adds the phosphate to the organic molecules such as carbohydrates, proteins, and lipids; phosphorylase adds phosphate to monomers like glycogen, starch, etc.
Kinases include the lipid kinases, protein kinases, and carbohydrates kinases; conversely, phosphorylases include the starch phosphorylase, glycogen phosphorylase, and polynucleotide phosphorylase.
Janet White
Apr 28, 2020
Kinase involves in the proteins, lipids kinases, whereas phosphorylase involves glycogen, starch phosphorylase.
Samantha Walker
Apr 28, 2020
Kinase uses ATP; phosphorylase uses inorganic phosphate as a source of phosphate on a substrate.

Comparison Chart


Phosphorylation takes place by the addition of phosphate from the high energy ATP, is known as a kinase.
Phosphorylation occurs from the transfer of phosphate from the inorganic phosphate, is known as phosphorylase.


In 1954s
In the late 1930s

Phosphate Source

ATP (organic chemical)
Inorganic phosphate(Pi)

Bond Breaking

Don’t break bonds of the substrate
Break the bonds of the substrate


Organic molecules (glucose, proteins, lipids, etc.)
Monomeric molecules as glucose, lactose
Janet White
Apr 28, 2020

PH Range

About 7.4, also at 5.0
From 6-7
Aimie Carlson
Apr 28, 2020


Protein kinases, lipids kinases, carbs kinases, etc.
Glycogen phosphorylases etc.

Kinase and Phosphorylase Definitions


Any of various enzymes that catalyze the transfer of a phosphate group from a donor, such as ADP or ATP, to an acceptor protein. Kinases regulate many essential cellular processes.


Any of a class of enzymes that catalyze the attachment of a phosphate group to another molecule.


Any of a group of enzymes that transfer phosphate groups from high-energy donor molecules, such as ATP, to specific target molecules (substrates), in a process termed phosphorylation.


(enzyme) Any enzyme that catalyzes the production of glucose phosphate from glycogen and inorganic phosphate


One of a class of enzymes that catalyze transfer of a phosphate group from ATP to another molecule; it is a type of phosphorylase.


An enzyme that catalyzes the conversion of a proenzyme to an active enzyme

Kinase vs. Phosphorylase

The kinase was first observed in the liver as protein kinase by the Gene Kennedy in 1954, whereas phosphorylase was observed by the Earl W. Sutherland Jr. in the late 1930s as glycogen phosphorylase.

When the donating agency is the high energy ATP molecule, the enzyme involved is a kinase, and when the donating agency is the inorganic phosphate as PO43- from H3PO4 (phosphoric acid) as a phosphorylation source then enzyme involved is phosphorylase. The kinase acceptors are organic molecules like carbohydrates, lipids, and proteins, etc. while the phosphorylase acceptors are specific substances or monomeric subunits like glycogen, starch, etc.

Kinases are not involved in the bond-breaking of a substrate during the transfer of the phosphate group, while the phosphorylase breaks the bonds of the substrate and specific substance by adding the phosphate group. The typical kind of kinase is protein kinase among the carbohydrates and lipids kinases, while the common type of phosphorylase is the glycogen phosphorylase.

What is Kinase?

Kinase in the form of the biological catalyst is one of the kinds of phosphotransferase which catalyze the phosphorylation by the transfer of phosphate group from the inorganic phosphate (PO43-). The kinase acts in various enzymatic reactions, not involved in the bond-breaking of the substrate.

Kinases play the role as a phosphotransferase, which regulates the functions of cells by transfer of phosphate from the donor ATP to substrate molecules such as glucose, proteins, and lipids as well. Kinase doesn’t break the bonds of substrate molecules on which they act during the transfer of phosphate groups. Some of the substrates are riboflavin, creatine, etc. Kinase acts as transmitting signals source regulating the complex processes in cells.

Classification of Kinase

  • Protein Kinase: One of the diverse and most significant functional family of kinases is a protein kinase. They deal with the functions and activity of many proteins by adding the phosphates to amino acids such as tyrosine, threonine, serine, and also histidine. P13K combines phosphorus with oxygen.
  • Lipid Kinase: These acts the reactivity of substrate lipids by the addition of phosphate as phosphoinositide three kinases involved in causing cancer when this lipid kinase act over the catalytic subunit PIK3CA (provides instructions for making p110 alpha protein).
  • Carbohydrate Kinase: These kinases act over the substrate carbohydrates such as glucose and hexokinase (an enzyme used during the glycolysis) regulating various metabolic pathways.

What is Phosphorylase?

Phosphorylase is also one of the kinds of phosphotransferase catalyzing phosphorylation from inorganic phosphates (Pi) to acceptor molecules. Phosphorylase enzymes are involved in the breaking of bonds of substrate and monomer molecule, unlike kinase by phosphate (PO43- ). It is regulated by the phosphorylation and also the allosteric regulation (binding of the enzyme other than the enzyme’s active site on effector molecule).

Phosphorylase is the active form in the activation sense of enzymes than the phosphorylase b. In the process of phosphorylation, ATP is required by the process, whereas in dephosphorylation, free inorganic phosphates are released as Pi. Phosphorylases are different from the phosphatases which remove the phosphate group from the donor by water. Phosphorylases also include the allosteric enzymes (can change their shape on binding of the effector, which play the role of catalyst for the production of glucose-1-phosphate from the glucan such as glycogen, starch, etc.

They are named accordingly by the addition of a substrate name with the phosphorylase as glycogen/starch phosphorylase is named as when substrate starch is acted by phosphorylase during the addition of phosphate from an inorganic phosphate.

Examples of phosphorylase are trehalose phosphorylase, glycogen/starch phosphorylase ( In glycogenesis), polynucleotide phosphorylase. The enzymatic disorders caused by phosphorylase are Glycogen storage disease type V-muscle glycogen and Glycogen storage disease type VI-liver glycogen.

Classification of Phosphorylase

  • Glycosyl Phosphotransferase: Enzymes removing glucose from glucans, e.g., maltodextrin phosphorylase, starch phosphorylase. Enzymes are breaking nucleosides into subunits of bases and sugars, e.g., purine nucleosides phosphorylase.
  • Nucleotidyl Phosphotransferase: Having activity of exonuclease 3’ to 5’ position, e.g., polynucleotide phosphorylated.

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