Stain vs. Dye in Histology
Main DifferenceThe main difference between stain and dye in histology is that stain is usually a combination of numerous dyes, whereas dye is a substance that highlights extracellular or intracellular elements of a tissue.
Difference Between Stain and Dye in Histology
Stain vs. Dye in Histology
Stain usually highlights different components in different colors, while dye only highlights a particular component of the tissue only in one color.
Stain vs. Dye in Histology
The stain is a combination of different dyes, whereas dye is a chemical reagent.
Stain vs. Dye in Histology
The main role of stain is to give contrast to the tissue; on the other hand, the main role of dye is to highlight a particular component within a tissue.
Stain vs. Dye in Histology
Stain normally used for biological specimens; conversely, the dye is used for normal purposes like staining clothes.
Stain vs. Dye in Histology
The stain is a purified form, and it does not contain impurities; on the contrary, a dye is a crude form of color that contains impurities.
Stain vs. Dye in Histology
The stain is produced with great attention or precautions and with extra fine conditions.
Stain vs. Dye in Histology
Various examples of stain are toluidine blue, Wright’s stain, H&E, Masson’s trichrome stain; on the flip side, some examples of dye are pyronin G, Aniline blue, Methyl green, orange G.
Stainnoun
A discoloured spot or area.
Stainnoun
A blemish on one's character or reputation.
Stainnoun
A substance used to soak into a surface and colour it.
Stainnoun
A reagent or dye used to stain microscope specimens so as to make some structures visible.
Stainnoun
(heraldry) Any of a number of non-standard tinctures used in modern heraldry.
Stainverb
(transitive) To discolour.
to stain the hand with dyearmour stained with bloodStainverb
To taint or tarnish someone's character or reputation
Stainverb
To coat a surface with a stain
to stain wood with acids, coloured washes, paint rubbed in, etc.the stained glass used for church windowsStainverb
(intransitive) To become stained; to take a stain.
Stainverb
To treat (a microscopic specimen) with a dye, especially one that dyes specific features
Stainverb
To cause to seem inferior or soiled by comparison.
Stainnoun
a soiled or discolored appearance;
the wine left a dark stainStainnoun
(microscopy) a dye or other coloring material that is used in microscopy to make structures visible
Stainnoun
the state of being covered with unclean things
Stainnoun
a symbol of disgrace or infamy;
And the Lord set a mark upon CainStainnoun
an act that brings discredit to the person who does it;
he made a huge blot on his copybookStainverb
color with a liquid dye or tint;
Stain this table a beautiful walnut colorpeople knew how to stain glass a beautiful blue in the middle agesStainverb
produce or leave stains;
Red wine stains the table clothStainverb
make dirty or spotty, as by exposure to air; also used metaphorically;
The silver was tarnished by the long exposure to the airHer reputation was sullied after the affair with a married manStainverb
color for microscopic study;
The laboratory worker dyed the specimenComparison Chart
| Stain | Dye |
| The stain is a mixture of specific dyes to color a biological synthetics in a lab. | The dye is a distinct chemical mixture present in a stain. |
| Significance | |
| A mixture of different dyes | A chemical reagent |
| Colors | |
| Highlights different works in different colors | Only highlights a particular component of the tissue only in one color |
| Role | |
| To give contrast to the tissue | Highlight a particular component within a tissue |
| Functions | |
| Normally used for biological specimens | Used for normal purposes like staining clothes |
| Purity/Impurity | |
| A purified form and it does not contain impurities in its structure | A crude form of color that contains impurities |
| Manufacture | |
| Manufactured with great care and under more rigid specifications | A crude form |
| Examples | |
| Toluidine blue, Wright’s stain, H&E, Masson’s trichrome stain | Pyronin G, Aniline blue, Methyl green, orange G |
Stain vs. Dye in Histology
Stain highlights different works in different colors, while dye only highlights a particular component of the tissue only in one color. The stain is a mixture of different dyes, whereas dye is a chemical reagent. The main role of stain is to give contrast to the tissue; on the other hand, the main role of dye is to highlight a particular component within a tissue. Stain normally used for biological specimens; conversely, dye is used for normal purposes like staining clothes. Stain is a purified form, and it does not contain impurities in its structure; on the contrary, a dye is a crude form of color that contains impurities. The stain is produced with great attention or precautions and with extra firm conditions, while the dye is a crude form. Various examples of stain are toluidine blue, Wright’s stain, H&E, Masson’s trichrome stain; on the flip side, some examples of dye are pyronin G, Aniline blue, Methyl green, orange G.
What is Stain?
The stain is generally a mixture of various dyes that enhance the contrast of biological specimen under in microscopic image. Normally, most cells that makeup tissues are transparent and colorless. That’s why to make the cells observable when observing under a microscope the tissues are stained in a specific way. Various chemical reagents are used in the technique of staining are the stains. Each dye present inside the stain is specifically accentuated a particular chemical object within a tissue. Hence, many constituents of tissues can be seen and observe in various contrasting colors under the microscope. Therefore, it means that stains give a contrasting shade to the microscopic slides and biological specimen. Stain process is not just limited for giving contrasting colors to biological specimen but can also be used to examine the structure of many other materials such as the spherical structures of block copolymers and the lamellar structure of partial-crystalline polymers. The stain is a purified form, and it does not contain impurities in its structure. The stain is produced with great attention or precautions and with extra fine conditions.
Types of Stain
- In Vivo Stain: In vivo stain is the method used to stain or dyeing living tissues.
- In Vitro Stain: In vitro stain is the process which includes the coloring of structures or cells that have been removed, or in other words, it is used to stain non-living tissues.
Examples
Various examples of stain are toluidine blue, Wright’s stain, H&E, Masson’s trichrome stain.
What is Dye?
A dye is a generally a single component which is contained in a strain. A particular dye can be used to accentuate a specific chemical object within a tissue. Hence, to accentuated many constituents in the same slide, then a mixture of several types of dyes should be used. Dye is a crude form of color that contains impurities. The dye is used for normal purposes like staining clothes. The two general central types of dye are acidic dye and basic dye. Basic dye usually deals with acidic/anionic constituents of the tissue, for instance, a nucleic acid. Acidic dye normally reacts with basic/cationic components of the tissue, for instance, proteins.
Basic Dye
- Methylene Blue: Blue color
- Pyronin G: Red color
- Toluidine Blue: Blue color
- Methyl Green: Green color
Acidic Dye
- Aniline Blue: Blue color
- Eosin: Red color
- Acid fuchsin: Red color
- Orange G: Orange color
ConclusionAbove discussion concludes that the stain is usually a mixture of several dyes and it is used to give contrast to the tissue, whereas dye is a substance that highlights extracellular or intracellular elements of tissue and used for usual purposes like staining clothes.
